Rapid construction of mechanically-confined multi-cellular structures
using dendrimeric intercellular linker
Xuejun Mo, Qiushi Li, Lena Wai, Yi Lui, Baixue Zheng, Chiang Huen Kang, Bramasta Nugrah, Zhilian Yue, Rui Rui Jia, Hong Xia Fu, Deepak Choudhury, Talha Arooz, Jie Yan, Chwee Teck Lim, Shali Sheng, Choon-Hong Tan, HanryYu
Biomaterials, 2010, 31, 7455 – 7467.
Abstract: Tissue constructs that mimic the in vivo cell–cell and cell–matrix interactions are especially useful for applications involving the cell- dense and matrix- poor internal organs. Rapid and precise arrangement of cells into functional tissue constructs remains a challenge in tissue engineering. We demonstrate rapid assembly of C3A cells into multi- cell structures using a dendrimeric intercellular linker. The linker is composed of oleyl- polyethylene glycol (PEG) derivatives conjugated to a 16 arms- polypropylenimine hexadecaamine (DAB) dendrimer. The positively charged multivalent dendrimer concentrates the linker onto the negatively charged cell surface to facilitate efficient insertion of the hydrophobic oleyl groups into the cellular membrane. Bringing linker- treated cells into close proximity to each other via mechanical means such as centrifugation and micromanipulation enables their rapid assembly into multi- cellular structures within minutes. The cells exhibit high levels of viability, proliferation, three- dimensional (3D) cell morphology and other functions in the constructs. We constructed defined multi- cellular structures such as rings, sheets or branching rods that can serve as potential tissue building blocks to be further assembled into complex 3D tissue constructs for biomedical applications.
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